Nanogels (NG) hold great promise as a drug delivery platform. In this work, we examine the potential of lysozyme-dextran nanogels (LDNG) as drug carriers in vitro using two cell lines: a model target tissue, human umbilical cord vein endothelial cells (HUVEC) and a model of the mononuclear phagocyte system (phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 cells). The LDNG (∼100 nm) were prepared with rhodamine-label dextran (LRDNG) via Maillard reaction followed by heat-gelation reaction and were loaded with a fluorescent probe, 5-hexadecanoylaminofluorescein (HAF), as a mock drug. Epifluorescence microscopy confirmed rapid uptake of LRDNG by HUVEC. Although LysoTracker Green staining indicated a lysosomal fate for LRDNG, the mock drug cargo (HAF) diffused extensively inside the cell within 15 min. Flow cytometry and confocal microscopy indicated slow uptake of LRDNG in PMA-stimulated THP-1 cells, with only 41% of cells containing LRDNG after 24 h exposure. Finally, 24 h exposure to LRDNG did not affect the viability of either cell type at the dose studied (20 μg/ml). At a higher dose (200 μg/ml), LRDNG resulted in a marked loss of viability of HUVEC and THP-1, measuring 30% and 38%, respectively. Collectively, our results demonstrate the great potential of LRDNG as a drug delivery platform, combining simple production, rapid uptake and cargo release in target cells with “stealth” properties and low cytotoxicity.
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February 2013
Research-Article
Cellular Uptake and Intracellular Cargo Release From Dextran Based Nanogel Drug Carriers
M. Carme Coll Ferrer,
M. Carme Coll Ferrer
1
Department of Anesthesiology and Critical Care
and Department of Materials Science and Engineering,
Philadelphia, PA 19104
and Department of Materials Science and Engineering,
University of Pennsylvania
,Philadelphia, PA 19104
1These authors contributed equally to this work.
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Peter Sobolewski,
Peter Sobolewski
1
Department of Anesthesiology and Critical Care,
University of Pennsylvania
,Philadelphia
, PA 19104
1These authors contributed equally to this work.
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Russell J. Composto,
Russell J. Composto
Department of Materials Science and Engineering,
Philadelphia, PA 19104
University of Pennsylvania
,Philadelphia, PA 19104
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David M. Eckmann
David M. Eckmann
2
Department of Anesthesiology and Critical Care,
e-mail: eckmanndm@uphs.upenn.edu
University of Pennsylvania
,Philadelphia
,PA 19104
e-mail: eckmanndm@uphs.upenn.edu
2Corresponding author.
Search for other works by this author on:
M. Carme Coll Ferrer
Department of Anesthesiology and Critical Care
and Department of Materials Science and Engineering,
Philadelphia, PA 19104
and Department of Materials Science and Engineering,
University of Pennsylvania
,Philadelphia, PA 19104
Peter Sobolewski
Department of Anesthesiology and Critical Care,
University of Pennsylvania
,Philadelphia
, PA 19104
Russell J. Composto
Department of Materials Science and Engineering,
Philadelphia, PA 19104
University of Pennsylvania
,Philadelphia, PA 19104
David M. Eckmann
Department of Anesthesiology and Critical Care,
e-mail: eckmanndm@uphs.upenn.edu
University of Pennsylvania
,Philadelphia
,PA 19104
e-mail: eckmanndm@uphs.upenn.edu
1These authors contributed equally to this work.
2Corresponding author.
Manuscript received September 11, 2012; final manuscript received December 10, 2012; published online July 11, 2013. Assoc. Editor: Malisa Sarntinoranont.
J. Nanotechnol. Eng. Med. Feb 2013, 4(1): 011002 (8 pages)
Published Online: July 11, 2013
Article history
Received:
September 11, 2012
Revision Received:
December 10, 2012
Citation
Carme Coll Ferrer, M., Sobolewski, P., Composto, R. J., and Eckmann, D. M. (July 11, 2013). "Cellular Uptake and Intracellular Cargo Release From Dextran Based Nanogel Drug Carriers." ASME. J. Nanotechnol. Eng. Med. February 2013; 4(1): 011002. https://doi.org/10.1115/1.4023246
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