Epithelial to mesenchymal transition (EMT) has been believed to play a critical role in cancer metastasis. has been described as an inducer of EMT in normal mammary epithelial cells by signaling through receptor serine/threonine kinase pathways to regulate epithelial cell plasticity and invasion. In this study, we investigated the EMT cellular responses, including morphologic changes, phenotype switches, invasiveness enhancement, and cellular contraction alteration, in stimulated human prostate normal epithelial cells (PZ-HPV-7). Migration of treated PZ-HPV-7 cells across matrigel was measured in invasion chambers ( pore size). The cells were treated with or without (2 ng/ml) in PrEGM media for 3 days. Immunoblot assay was conducted and it was demonstrated that the induction of vimentin when stimulated by was accompanied by a downregulation of E-cadherin, though p-cadherin level was not altered. It was also observed that there was a decrease in cytokaretin 5/6 expression associated with the downregulation of E-cadherin during the induction of EMT. In order to study the cell contraction, three-dimensional collage lattice assay was performed. It was demonstrated that -stimulated PZ-HPV-7 cells gained contractility. Our results showed that stimulation induced PZ-HPV-7 cells to undergo epithelial to mesenchymal transition. EMT characteristics such as acquisition of mesenchymal markers and loss of epithelial markers were evident in the induction of vimentin and downregulation of E-cadherin and cytokeratins, as well as phenotypic alterations including increased contraction and enhanced mobility were detected.
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e-mail: victor.lin@utsouthwestern.edu
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Research Papers
Molecular Characterization of Epithelial to Mesenchymal Transition in Human Prostatic Epithelial Cells
Victor K. Lin,
Victor K. Lin
Department of Urology,
e-mail: victor.lin@utsouthwestern.edu
University of Texas Southwestern Medical Center
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Shih-Ya Wang,
Shih-Ya Wang
Department of Urology,
University of Texas Southwestern Medical Center
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Lanxiao Wu,
Lanxiao Wu
Department of Bioengineering,
University of Texas at Arlington
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Smitha M. Rao,
Smitha M. Rao
Department of Electrical Engineering,
University of Texas at Arlington
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J. C. Chiao,
J. C. Chiao
Department of Electrical Engineering,
University of Texas at Arlington
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Claus G. Roehrborn
Claus G. Roehrborn
Department of Urology,
University of Texas Southwestern Medical Center
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Victor K. Lin
Department of Urology,
University of Texas Southwestern Medical Center
e-mail: victor.lin@utsouthwestern.edu
Shih-Ya Wang
Department of Urology,
University of Texas Southwestern Medical Center
Lanxiao Wu
Department of Bioengineering,
University of Texas at Arlington
Smitha M. Rao
Department of Electrical Engineering,
University of Texas at Arlington
J. C. Chiao
Department of Electrical Engineering,
University of Texas at Arlington
Claus G. Roehrborn
Department of Urology,
University of Texas Southwestern Medical Center
J. Nanotechnol. Eng. Med. May 2010, 1(2): 021011 (6 pages)
Published Online: May 14, 2010
Article history
Received:
March 16, 2010
Revised:
March 31, 2010
Online:
May 14, 2010
Published:
May 14, 2010
Citation
Lin, V. K., Wang, S., Wu, L., Rao, S. M., Chiao, J. C., and Roehrborn, C. G. (May 14, 2010). "Molecular Characterization of Epithelial to Mesenchymal Transition in Human Prostatic Epithelial Cells." ASME. J. Nanotechnol. Eng. Med. May 2010; 1(2): 021011. https://doi.org/10.1115/1.4001542
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