Abstract
Single-crystalline nanoporous gallium nitride (GaN) thin films were fabricated with the pore size readily tunable in 20–100 nm. Uniform adhesion and spreading of human mesenchymal stem cells (hMSCs) seeded on these thin films peak on the surface with pore size of 30 nm. Substantial cell elongation emerges as pore size increases to ∼80 nm. The osteogenic differentiation of hMSCs occurs preferentially on the films with 30 nm sized nanopores, which is correlated with the optimum condition for cell spreading, which suggests that adhesion, spreading, and stem cell differentiation are interlinked and might be coregulated by nanotopography.
Issue Section:
Research Papers
References
1.
Collins
,
R. T.
,
Fauchet
,
P. M.
, and
Tischler
,
M. A.
, 1997
, “
Porous Silicon: From Luminescence to LEDs
,” Phys. Today
,
50
(1
), pp. 24
–31
.10.1063/1.8816502.
Lazarouk
,
S.
,
Jaguiro
,
P.
,
Katsouba
,
S.
,
Masini
,
G.
,
La Monica
,
S.
,
Maiello
,
G.
, and
Ferrari
,
A.
, 1996
, “
Stable Electroluminescence From Reverse Biased n-Type Porous Silicon-Aluminum Schottky Junction Device
,” Appl. Phys. Lett.
,
68
(15
), pp. 2108
–2110
.10.1063/1.1156003.
Richter
,
A.
,
Steiner
,
P.
,
Kozlowski
,
F.
, and
Lang
,
W.
, 1991
, “
Current-Induced Light-Emission From a Porous Silicon Device
,” IEEE Electron Device Lett.
,
12
(12
), pp. 691
–692
.10.1109/55.1169574.
Steiner
,
P.
,
Kozlowski
,
F.
, and
Lang
,
W.
, 1993
, “
Light-Emitting Porous Silicon Diode With an Increased Electroluminescence Quantum Efficiency
,” Appl. Phys. Lett.
,
62
(21
), pp. 2700
–2702
.10.1063/1.1092365.
Foucaran
,
A.
,
Pascal-Delannoy
,
F.
,
Giani
,
A.
,
Sackda
,
A.
,
Combette
,
P.
, and
Boyer
,
A.
, 1997
, “
Porous Silicon Layers Used for Gas Sensor Applications
,” Thin Solid Films
,
297
(1–2
), pp. 317
–320
.10.1016/S0040-6090(96)09437-06.
Sailor
,
M. J.
, and
Link
,
J. R.
, 2005
, “
‘Smart Dust’: Nanostructured Devices in a Grain of Sand
,” Chem. Commun.
,
21
(11
), pp. 1375
–1383
.10.1039/B417554A7.
Chan
,
S.
,
Fauchet
,
P. M.
,
Li
,
Y.
,
Rothberg
,
L. J.
, and
Miller
,
B. L.
, 2000
, “
Porous Silicon Microcavities for Biosensing Applications
,” Phys. Status Solidi A
,
182
(1
), pp. 541
–546
.10.1002/1521-396X(200011)182:1<541::AID-PSSA541>3.0.CO;2-#8.
Coffer
,
J. L.
,
Whitehead
,
M. A.
,
Nagesha
,
D. K.
,
Mukherjee
,
P.
,
Akkaraju
,
G.
,
Totolici
,
M.
,
Saffie
,
R. S.
, and
Canham
,
L. T.
, 2005
, “
Porous Silicon-Based Scaffolds for Tissue Engineering and Other Biomedical Applications
,” Phys. Status Solidi A
,
202
(8
), pp. 1451
–1455
.10.1002/pssa.2004611349.
Lin
,
V. S. Y.
,
Motesharei
,
K.
,
Dancil
,
K. P.
,
Sailor
,
M. J.
, and
Ghadiri
,
M. R.
, 1997
, “
A Porous Silicon-Based Optical Interferometric Biosensor
,” Science
,
278
(5339
), pp. 840
–843
.10.1126/science.278.5339.84010.
Thust
,
M.
,
Schöning
,
M. J.
,
Frohnhoff
,
S.
,
Arens-Fischer
,
R.
,
Kordos
,
P.
, and
Lüth
,
H.
, 1996
, “
Porous Silicon as a Substrate Material for Potentiometric Biosensors
,” Meas. Sci. Technol.
,
7
(1
), pp. 26
–29
.10.1088/0957-0233/7/1/00311.
Dancil
,
K. P. S.
,
Greiner
,
D. P.
, and
Sailor
,
M. J.
, 1999
, “
A Porous Silicon Optical Biosensor: Detection of Reversible Binding of IgG to a Protein A-Modified Surface
,” J. Am. Chem. Soc.
,
121
(34
), pp. 7925
–7930
.10.1021/ja991421n12.
Haidary
,
S. M.
,
Corcoles
,
E. P.
, and
Ali
,
N. K.
, 2012
, “
Nanoporous Silicon as Drug Delivery Systems for Cancer Therapies
,” J. Nanomater.
,
2012
, p. 830503
.10.1155/2012/83050313.
Ileri
,
N.
,
Létant
,
S. E.
,
Britten
,
J.
,
Nguyen
,
H.
,
Larson
,
C.
,
Zaidi
,
S.
,
Palazoglu
,
A.
,
Faller
,
R.
,
Tringe
,
J. W.
, and
Stroeve
,
P.
, 2009
, “
Efficient Nanoporous Silicon Membranes for Integrated Microfluidic Separation and Sensing Systems
,” MRS Proc.
,
1191
, pp. 87
–92
.10.1557/PROC-1191-OO09-0214.
Kim
,
S. T.
,
Kim
,
D.-J.
,
Kim
,
T.-J.
,
Seo
,
D.-W.
,
Kim
,
T.-H.
,
Lee
,
S.-Y.
,
Kim
,
K.
,
Lee
,
K.-M.
, and
Lee
,
S.-K.
, 2010
, “
Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4(+) T Lymphocyte Separation
,” Nano Lett.
,
10
(8
), pp. 2877
–2883
.10.1021/nl100942p15.
Lee
,
S. K.
,
Kim
,
G.-S.
,
Wu
,
Y.
,
Kim
,
D.-J.
,
Lu
,
Y.
,
Kwak
,
M.
,
Han
,
L.
,
Hyung
,
J.-H.
,
Seol
,
J.-K.
,
Sander
,
C.
,
Gonzalez
,
A.
,
Li
,
J.
, and
Fan
,
R.
, 2012
, “
Nanowire Substrate-Based Laser Scanning Cytometry for Quantitation of Circulating Tumor Cells
,” Nano Lett.
,
12
(6
), pp. 2697
–2704
.10.1021/nl204170716.
Shalek
,
A. K.
,
Robinson
,
J. T.
,
Karp
,
E. S.
,
Lee
,
J. S.
,
Ahn
,
D.-R.
,
Yoon
,
M.-H.
,
Sutton
,
A.
,
Jorgolli
,
M.
,
Gertner
,
R. S.
,
Gujral
,
T. S.
,
MacBeath
,
G.
,
Yang
,
E. G.
, and
Park
,
H.
, 2010
, “
Vertical Silicon Nanowires as a Universal Platform for Delivering Biomolecules Into Living Cells
,” Proc. Natl. Acad. Sci. U. S. A.
,
107
(5
), pp. 1870
–1875
.10.1073/pnas.090935010717.
Andersson
,
A. S.
,
Bäckhed
,
F.
,
von Euler
,
A.
,
Richter-Dahlfors
,
A.
,
Sutherland
,
D.
, and
Kasemo
,
B.
, 2003
, “
Nanoscale Features Influence Epithelial Cell Morphology and Cytokine Production
,” Biomaterials
,
24
(20
), pp. 3427
–3436
.10.1016/S0142-9612(03)00208-418.
Dalby
,
M. J.
,
Riehle
,
M. O.
,
Johnstone
,
H. J.
,
Affrossman
,
S.
, and
Curtis
,
A. S.
, 2002
, “
Polymer-Demixed Nanotopography: Control of Fibroblast Spreading and Proliferation
,” Tissue Eng.
,
8
(6
), pp. 1099
–1108
.10.1089/10763270232093419119.
Dalby
,
M. J.
,
Yarwood
,
S. J.
,
Riehle
,
M. O.
,
Johnstone
,
H. J.
,
Affrossman
,
S.
, and
Curtis
,
A. S.
, 2002
, “
Increasing Fibroblast Response to Materials Using Nanotopography: Morphological and Genetic Measurements of Cell Response to 13-nm-High Polymer Demixed Islands
,” Exp. Cell Res.
,
276
(1
), pp. 1
–9
.10.1006/excr.2002.549820.
Rice
,
J. M.
,
Hunt
,
J. A.
,
Gallagher
,
J. A.
,
Hanarp
,
P.
,
Sutherland
,
D. S.
, and
Gold
,
J.
, 2003
, “
Quantitative Assessment of the Response of Primary Derived Human Osteoblasts and Macrophages to a Range of Nanotopography Surfaces in a Single Culture Model In Vitro
,” Biomaterials
,
24
(26
), pp. 4799
–4818
.10.1016/S0142-9612(03)00381-821.
Riehle
,
M. O.
,
Dalby
,
M. J.
,
Johnstone
,
H.
,
MacIntosh
,
A.
, and
Affrossman
,
S.
, 2003
, “
Cell Behaviour of Rat Calvaria Bone Cells on Surfaces With Random Nanometric Features
,” Mater. Sci. Eng., C
,
23
(3
), pp. 337
–340
.10.1016/S0928-4931(02)00282-522.
Dalby
,
M. J.
,
Gadegaard
,
N.
,
Riehle
,
M. O.
,
Wilkinson
,
C. D.
, and
Curtis
,
A. S.
, 2004
, “
Investigating Filopodia Sensing Using Arrays of Defined Nano-Pits Down to 35 nm Diameter in Size
,” Int. J. Biochem. Cell Biol.
,
36
(10
), pp. 2005
–2015
.10.1016/j.biocel.2004.03.00123.
Dalby
,
M. J.
,
Riehle
,
M. O.
,
Johnstone
,
H. J.
,
Affrossman
,
S.
, and
Curtis
,
A. S.
, 2003
, “
Nonadhesive Nanotopography: Fibroblast Response to Poly(n-Butyl Methacrylate)-Poly(Styrene) Demixed Surface Features
,” J. Biomed. Mater. Res., Part A
,
67A
(3
), pp. 1025
–1032
.10.1002/jbm.a.1013924.
Goldberg
,
D. J.
, and
Burmeister
,
D. W.
, 1986
, “
Stages in Axon Formation-Observations of Growth of Aplysia Axons in Culture Using Video-Enhanced Contrast-Differential Interference Contrast Microscopy
,” J. Cell Biol.
,
103
(5
), pp. 1921
–1931
.10.1083/jcb.103.5.192125.
Polinsky
,
M.
,
Balazovich
,
K.
, and
Tosney
,
K. W.
, 2000
, “
Identification of an Invariant Response: Stable Contact With Schwann Cells Induces Veil Extension in Sensory Growth Cones
,” J. Neurosci.
,
20
(3
), pp. 1044
–1055
.26.
Yim
,
E. K. F.
,
Reano
,
R. M.
,
Pang
,
S. W.
,
Yee
,
A. F.
,
Chen
,
C. S.
, and
Leong
,
K. W.
, 2005
, “
Nanopattern-Induced Changes in Morphology and Motility of Smooth Muscle Cells
,” Biomaterials
,
26
(26
), pp. 5405
–5413
.10.1016/j.biomaterials.2005.01.05827.
Dalby
,
M. J.
,
Gadegaard
,
N.
,
Tare
,
R.
,
Andar
,
A.
,
Riehle
,
M. O.
,
Herzyk
,
P.
,
Wilkinson
,
C. D. W.
, and
Oreffo
,
R. O. C.
, 2007
, “
The Control of Human Mesenchymal Cell Differentiation Using Nanoscale Symmetry and Disorder
,” Nat. Mater.
,
6
(12
), pp. 997
–1003
.10.1038/nmat201328.
Yim
,
E. K. F.
,
Pang
,
S. W.
, and
Leong
,
K. W.
, 2007
, “
Synthetic Nanostructures Inducing Differentiation of Human Mesenchymal Stem Cells Into Neuronal Lineage
,” Exp. Cell Res.
,
313
(9
), pp. 1820
–1829
.10.1016/j.yexcr.2007.02.03129.
Oh
,
S.
,
Brammer
,
K. S.
,
Li
,
Y. S.
,
Teng
,
D.
,
Engler
,
A. J.
,
Chien
,
S.
, and
Jin
,
S.
, 2009
, “
Stem Cell Fate Dictated Solely by Altered Nanotube Dimension
,” Proc. Natl. Acad. Sci. U. S. A.
,
106
(7
), pp. 2130
–2135
.10.1073/pnas.081320010630.
McMurray
,
R. J.
,
Gadegaard
,
N.
,
Tsimbouri
,
P. M.
,
Burgess
,
K. V.
,
McNamara
,
L. E.
,
Tare
,
R.
,
Murawski
,
K.
,
Kingham
,
E.
,
Oreffo
,
R. O. C.
, and
Dalby
,
M. J.
, 2011
, “
Nanoscale Surfaces for the Long-Term Maintenance of Mesenchymal Stem Cell Phenotype and Multipotency
,” Nat. Mater.
,
10
(8
), pp. 637
–644
.10.1038/nmat305831.
Yim
,
E. K. F.
,
Darling
,
E. M.
,
Kulangara
,
K.
,
Guilak
,
F.
, and
Leong
,
K. W.
, 2010
, “
Nanotopography-Induced Changes in Focal Adhesions, Cytoskeletal Organization, and Mechanical Properties of Human Mesenchymal Stem Cells
,” Biomaterials
,
31
(6
), pp. 1299
–1306
.10.1016/j.biomaterials.2009.10.03732.
Bauer
,
S.
,
Park
,
J.
,
Faltenbacher
,
J.
,
Berger
,
S.
,
von der Mark
,
K.
, and
Schmuki
,
P.
, 2009
, “
Size Selective Behavior of Mesenchymal Stem Cells on ZrO2 and TiO2 Nanotube Arrays
,” Integr. Biol.
,
1
(8–9
), pp. 525
–532
.10.1039/b908196h33.
Webster
,
T. J.
,
Ergun
,
C.
,
Doremus
,
R. H.
,
Siegel
,
R. W.
, and
Bizios
,
R.
, 2000
, “
Enhanced Functions of Osteoblasts on Nanophase Ceramics
,” Biomaterials
,
21
(17
), pp. 1803
–1810
.10.1016/S0142-9612(00)00075-234.
Popat
,
K. C.
,
Chatvanichkul
,
K. I.
,
Barnes
,
G. L.
,
Latempa
,
T. J.
, Jr.
,
Grimes
,
C. A.
, and
Desai
,
T. A.
, 2007
, “
Osteogenic Differentiation of Marrow Stromal Cells Cultured on Nanoporous Alumina Surfaces
,” J. Biomed. Mater. Res., Part A
,
80A
(4
), pp. 955
–964
.10.1002/jbm.a.3102835.
Kim
,
J. A.
,
Jang
,
E. Y.
,
Kang
,
T. J.
,
Yoon
,
S.
,
Ovalle-Robles
,
R.
,
Rhee
,
W. J.
,
Kim
,
T.
,
Baughman
,
R. H.
,
Kim
,
Y. H.
, and
Park
,
T. H.
, 2012
, “
Regulation of Morphogenesis and Neural Differentiation of Human Mesenchymal Stem Cells Using Carbon Nanotube Sheets
,” Integr. Biol.
,
4
(6
), pp. 587
–594
.10.1039/c2ib20017a36.
Kim
,
S. J.
,
Lee
,
J. K.
,
Kim
,
J. W.
,
Jung
,
J. W.
,
Seo
,
K.
,
Park
,
S. B.
,
Roh
,
K. H.
,
Lee
,
S. R.
,
Hong
,
Y. H.
,
Kim
,
S. J.
,
Lee
,
Y. S.
,
Kim
,
S. J.
, and
Kang
,
K. S.
, 2008
, “
Surface Modification of Polydimethylsiloxane (PDMS) Induced Proliferation and Neural-Like Cells Differentiation of Umbilical Cord Blood-Derived Mesenchymal Stem Cells
,” J. Mater. Sci.: Mater. Med.
,
19
(8
), pp. 2953
–2962
.10.1007/s10856-008-3413-637.
Park
,
J.
,
Bauer
,
S.
,
von der Mark
,
K.
, and
Schmuki
,
P.
, 2007
, “
Nanosize and Vitality: TiO2 Nanotube Diameter Directs Cell Fate
,” Nano Lett.
,
7
(6
), pp. 1686
–1691
.10.1021/nl070678d38.
Jewett
,
S. A.
,
Makowski
,
M. S.
,
Andrews
,
B.
,
Manfra
,
M. J.
, and
Ivanisevic
,
A.
, 2012
, “
Gallium Nitride is Biocompatible and Non-Toxic Before and After Functionalization With Peptides
,” Acta Biomater.
,
8
(2
), pp. 728
–733
.10.1016/j.actbio.2011.09.03839.
Zhang
,
Y.
,
Ryu
,
S.-W.
,
Yerino
,
C.
,
Leung
,
B.
,
Sun
,
Q.
,
Song
,
Q.
,
Cao
,
H.
, and
Han
,
J.
, 2010
, “
A Conductivity-Based Selective Etching for Next Generation GaN Devices
,” Phys. Status Solidi B
,
247
(7
), pp. 1713
–1716
.10.1002/pssb.20098365040.
Zhang
,
Y.
,
Sun
,
Q.
,
Leung
,
B.
,
Simon
,
J.
,
Lee
,
M. L.
, and
Han
,
J.
, 2011
, “
The Fabrication of Large-Area, Free-Standing GaN by a Novel Nanoetching Process
,” Nanotechnology
,
22
(4
), p. 045603
.10.1088/0957-4484/22/4/04560341.
Cullis
,
A. G.
, and
Canham
,
L. T.
, 1991
, “
Visible-Light Emission Due to Quantum Size Effects in Highly Porous Crystalline Silicon
,” Nature
,
353
(6342
), pp. 335
–338
.10.1038/353335a0Copyright © 2014 by ASME
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