Bioprinting is a technique of creating 3D cell-laden structures by accurately dispensing biomaterial to form complex synthetic tissue. The printed constructs aim to mimic the native tissue by preserving the cell functionality and viability within the printed structure. The 3D bioprinting system presented in this paper aims to facilitate the process of 3D bioprinting through its ability to control the environmental parameters within an enclosed printing chamber. This design of the bioprinter targets to eliminate the need for a laminar flow hood, by regulating the necessary environmental conditions important for cell survival, especially during long duration prints. A syringe-based extrusion (SBE) deposition method comprising multiple nozzles is integrated into the system. This allows for a wider selection of biomaterials that can be used for the formation of the extracellular matrix (ECM). Tissue constructs composed of alginate-gelatin hydrogels were mixed with fibrinogen and human endothelial cells which were then characterized and compared using two methodologies: casted and bioprinted. Furthermore, vasculature was incorporated in the bioprinted constructs using sacrificial printing. Structural and functional characterization of the constructs were performed by assessing rheological, mechanical properties, and analyzing live-dead assay measurements.
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September 2019
Research-Article
Self-Contained Three-Dimensional Bioprinter for Applications in Cardiovascular Research
Prabhuti Kharel,
Prabhuti Kharel
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
e-mail: pkharel2013@my.fit.edu
Florida Institute of Technology,
Melbourne, FL 32901
e-mail: pkharel2013@my.fit.edu
1Corresponding author.
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Likitha Somasekhar,
Likitha Somasekhar
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
2P. Kharel and L. Somasekhar have contributed equally.
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Amy Vecheck,
Amy Vecheck
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
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Kunal Mitra
Kunal Mitra
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
Search for other works by this author on:
Prabhuti Kharel
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
e-mail: pkharel2013@my.fit.edu
Florida Institute of Technology,
Melbourne, FL 32901
e-mail: pkharel2013@my.fit.edu
Likitha Somasekhar
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
Amy Vecheck
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
Kunal Mitra
Biomedical Engineering,
Florida Institute of Technology,
Melbourne, FL 32901
Florida Institute of Technology,
Melbourne, FL 32901
1Corresponding author.
2P. Kharel and L. Somasekhar have contributed equally.
Manuscript received January 30, 2019; final manuscript received May 29, 2019; published online July 15, 2019. Assoc. Editor: Prasanna Hariharan.
J. Med. Devices. Sep 2019, 13(3): 031010 (7 pages)
Published Online: July 15, 2019
Article history
Received:
January 30, 2019
Revised:
May 29, 2019
Citation
Kharel, P., Somasekhar, L., Vecheck, A., and Mitra, K. (July 15, 2019). "Self-Contained Three-Dimensional Bioprinter for Applications in Cardiovascular Research." ASME. J. Med. Devices. September 2019; 13(3): 031010. https://doi.org/10.1115/1.4043960
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