Lectins (adhesins) on bacterial surfaces play important roles in infection by mediating bacterial adherence to host cell surfaces via their cognate receptors. We have explored the use of -D-mannose receptors as capturing agents for the detection of Escherichia coli using a microcantilever and have demonstrated that E. coli ORN178, which expresses normal type-1 pili, can interact with microcantilevers functionalized with -D-mannose and can cause shifts in its resonance frequencies. Although E. coli ORN208, which expresses abnormal pili, binds poorly to -D-mannose on the nitrocellulose membrane of a FAST slide, it did cause a detectable shift in resonance frequency when interacting with the -D-mannose functionalized microcantilevers.
Adhesin-Specific Nanomechanical Cantilever Biosensors for Detection of Microorganisms
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Tzeng, T. J., Cheng, Y. R., Saeidpourazar, R., Aphale, S. S., and Jalili, N. (September 30, 2010). "Adhesin-Specific Nanomechanical Cantilever Biosensors for Detection of Microorganisms." ASME. J. Heat Transfer. January 2011; 133(1): 011012. https://doi.org/10.1115/1.4002363
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