The present work investigates the effects of various guide vane designs on the heat transfer enhancement of rotating U-duct configuration with parallel 45-deg ribs. The ribs were installed on the bottom wall of the channel, which has a constant heat flux boundary condition. The channel has a square cross section with a 5.08 cm hydraulic diameter. The first and second passes are 514 mm and 460 mm, respectively. The range of Reynolds number for turbulent flow is up to 35,000. The channel rotates at various speeds up to 600 rpm, which brings the maximum rotation number of 0.75. Several computational fluid dynamics simulations are carried out for this study to understand the effect of guide vanes on flow and heat transfer in serpentine channels under various operating conditions.