Osteogenesis is the process by which mesenchymal stem cells differentiate to osteoblasts and form bone. The morphology and root mean squared (RMS) traction of four cell types representing different stages of osteogenesis were quantified. Undifferentiated D1, differentiated D1, MC3T3-E1, and MLO-A5 cell types were evaluated using both automated image analysis of cells stained for F-actin and by traction force microscopy (TFM). Undifferentiated mesenchymal stem cell lines were small, spindly, and exerted low traction, while differentiated osteoblasts were large, had multiple processes, and exerted higher traction. Size, shape, and traction all correlated with the differentiation stage. Thus, cell morphology evolved and RMS traction increased with differentiation. The results provide a foundation for further work with these cell lines to study the mechanobiology of bone formation.
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December 2015
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Differences in Morphology and Traction Generation of Cell Lines Representing Different Stages of Osteogenesis
Michael J. Poellmann,
Michael J. Poellmann
Mem. ASME
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
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Jonathan B. Estrada,
Jonathan B. Estrada
School of Engineering,
Brown University,
Providence, RI 02912
Brown University,
Providence, RI 02912
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Thomas Boudou,
Thomas Boudou
Laboratory of Materials and Physical Engineering,
Grenoble Institute of Technology,
Grenoble 38016, France
Grenoble Institute of Technology,
Grenoble 38016, France
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Zachary T. Berent,
Zachary T. Berent
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
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Amy J. Wagoner Johnson
Amy J. Wagoner Johnson
Mem. ASME
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
1206 W. Green Street,
Urbana, IL 61801
e-mail: ajwj@illinois.edu
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
1206 W. Green Street,
Urbana, IL 61801
e-mail: ajwj@illinois.edu
Search for other works by this author on:
Michael J. Poellmann
Mem. ASME
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
Jonathan B. Estrada
School of Engineering,
Brown University,
Providence, RI 02912
Brown University,
Providence, RI 02912
Thomas Boudou
Laboratory of Materials and Physical Engineering,
Grenoble Institute of Technology,
Grenoble 38016, France
Grenoble Institute of Technology,
Grenoble 38016, France
Zachary T. Berent
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
University of Illinois at Urbana-Champaign,
Urbana, IL 61801
Christian Franck
Amy J. Wagoner Johnson
Mem. ASME
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
1206 W. Green Street,
Urbana, IL 61801
e-mail: ajwj@illinois.edu
Department of Mechanical Science and Engineering,
University of Illinois at Urbana-Champaign,
1206 W. Green Street,
Urbana, IL 61801
e-mail: ajwj@illinois.edu
1Corresponding author.
Manuscript received April 10, 2015; final manuscript received October 10, 2015; published online November 3, 2015. Assoc. Editor: Carlijn V. C Bouten.
J Biomech Eng. Dec 2015, 137(12): 124503 (5 pages)
Published Online: November 3, 2015
Article history
Received:
April 10, 2015
Revised:
October 10, 2015
Citation
Poellmann, M. J., Estrada, J. B., Boudou, T., Berent, Z. T., Franck, C., and Wagoner Johnson, A. J. (November 3, 2015). "Differences in Morphology and Traction Generation of Cell Lines Representing Different Stages of Osteogenesis." ASME. J Biomech Eng. December 2015; 137(12): 124503. https://doi.org/10.1115/1.4031848
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