The aim of this study was to maximize oxygen diffusion within a three-dimensional scaffold in order to improve cell viability and proliferation. To evaluate the effect of pore architecture on oxygen diffusion, we designed a regular channel shape with uniform diameter, referred to as cylinder shaped, and a new channel shape with a channel diameter gradient, referred to as cone shaped. A numerical analysis predicted higher oxygen concentration in the cone-shaped channels than in the cylinder-shaped channels, throughout the scaffold. To confirm these numerical results, we examined cell proliferation and viability in 2D constructs and 3D scaffolds. Cell culture experiments revealed that cell proliferation and viability were superior in the constructs and scaffolds with cone-shaped channels.
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e-mail: dwcho@postech.ac.kr
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October 2010
Technical Briefs
Effect of Pore Architecture on Oxygen Diffusion in 3D Scaffolds for Tissue Engineering
Geunseon Ahn,
Geunseon Ahn
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
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Jeong Hun Park,
Jeong Hun Park
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
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Taeyun Kang,
Taeyun Kang
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
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Jin Woo Lee,
Jin Woo Lee
Research Associate
Department of Mechanical Engineering,
University of Texas
, Austin, TX 78712-0292
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Hyun-Wook Kang,
Hyun-Wook Kang
Research Associate
Wake Forest Institute for Regenerative Medicine,
Wake Forest University
, Medical Center Boulevard, Winston-Salem, NC 27157
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Dong-Woo Cho
Dong-Woo Cho
Professor
Mem. ASME Mem.TERMIS
Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology,
e-mail: dwcho@postech.ac.kr
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
Search for other works by this author on:
Geunseon Ahn
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
Jeong Hun Park
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
Taeyun Kang
Research Assistant
Department of Mechanical Engineering,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea
Jin Woo Lee
Research Associate
Department of Mechanical Engineering,
University of Texas
, Austin, TX 78712-0292
Hyun-Wook Kang
Research Associate
Wake Forest Institute for Regenerative Medicine,
Wake Forest University
, Medical Center Boulevard, Winston-Salem, NC 27157
Dong-Woo Cho
Professor
Mem. ASME Mem.TERMIS
Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology,
Pohang University of Science and Technology (POSTECH)
, San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Koreae-mail: dwcho@postech.ac.kr
J Biomech Eng. Oct 2010, 132(10): 104506 (5 pages)
Published Online: September 28, 2010
Article history
Received:
April 7, 2010
Revised:
August 3, 2010
Posted:
August 24, 2010
Published:
September 28, 2010
Online:
September 28, 2010
Citation
Ahn, G., Park, J. H., Kang, T., Lee, J. W., Kang, H., and Cho, D. (September 28, 2010). "Effect of Pore Architecture on Oxygen Diffusion in 3D Scaffolds for Tissue Engineering." ASME. J Biomech Eng. October 2010; 132(10): 104506. https://doi.org/10.1115/1.4002429
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