The following analysis presents an experimental and theoretical study of the passive viscoelastic behavior of human leukocytes. Individual neutrophils in EDTA were observed both during their partial aspiration into a small micropipette and after expulsion from a large micropipette where the cell had been totally aspirated and deformed into a sausage shape. To analyze the data, a passive model of leukocyte rheology has been developed consisting of a cortical shell containing a Maxwell fluid which describes the average properties of the cell cytoplasm. The cortical shell represents a crosslinked actin layer near the surface of the cell and is assumed to be under pre-stressed tension. This model can reproduce the results of experiments using micropipette for both short-time small deformation and slow recovery data after large deformation. In addition, a finite element scheme has been established for the same model which shows close agreement with the analytical solution.
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February 1988
Research Papers
Passive Deformation Analysis of Human Leukocytes
Cheng Dong,
Cheng Dong
Bioengineering Institute, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, NY 10027
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R. Skalak,
R. Skalak
Bioengineering Institute, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, NY 10027
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Kuo-Li Paul Sung,
Kuo-Li Paul Sung
Department of Physiology, College of Physicians & Surgeons, Columbia University New York, NY 10032
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G. W. Schmid-Scho¨nbein,
G. W. Schmid-Scho¨nbein
AMES–Bioengineering, University of California, San Diego, La Jolla, CA 92093
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Shu Chien
Shu Chien
Department of Physiology, College of Physicians & Surgeons, Columbia University, New York, NY 10032
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Cheng Dong
Bioengineering Institute, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, NY 10027
R. Skalak
Bioengineering Institute, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, NY 10027
Kuo-Li Paul Sung
Department of Physiology, College of Physicians & Surgeons, Columbia University New York, NY 10032
G. W. Schmid-Scho¨nbein
AMES–Bioengineering, University of California, San Diego, La Jolla, CA 92093
Shu Chien
Department of Physiology, College of Physicians & Surgeons, Columbia University, New York, NY 10032
J Biomech Eng. Feb 1988, 110(1): 27-36 (10 pages)
Published Online: February 1, 1988
Article history
Received:
November 16, 1987
Revised:
December 1, 1987
Online:
June 12, 2009
Citation
Dong, C., Skalak, R., Sung, K. P., Schmid-Scho¨nbein, G. W., and Chien, S. (February 1, 1988). "Passive Deformation Analysis of Human Leukocytes." ASME. J Biomech Eng. February 1988; 110(1): 27–36. https://doi.org/10.1115/1.3108402
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