While useful models have been proposed to predict the mechanical impact of damage in tendon and other soft tissues, the applicability of these models for describing in vivo injury and age-related degeneration has not been investigated. Therefore, the objective of this study was to develop and validate a simple damage model to predict mechanical alterations in mouse patellar tendons after aging, injury, or healing. To characterize baseline properties, uninjured controls at age 150 days were cyclically loaded across three strain levels and five frequencies. For comparison, damage was induced in mature (120 day-old) mice through either injury or aging. Injured mice were sacrificed at three or six weeks after surgery, while aged mice were sacrificed at either 300 or 570 days old. Changes in mechanical properties (relative to baseline) in the three week post-injury group were assessed and used to develop an empirical damage model based on a simple damage parameter related to the equilibrium stress at a prescribed strain (6%). From the derived model, the viscoelastic properties of the 300 day-old, 570 day-old, and six week post-injury groups were accurately predicted. Across testing conditions, nearly all correlations between predicted and measured parameters were statistically significant and coefficients of determination ranged from R2 = 0.25 to 0.97. Results suggest that the proposed damage model could exploit simple in vivo mechanical measurements to predict how an injured or aged tendon will respond to complex physiological loading regimens.
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e-mail: dbirk@health.usf.edu
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April 2013
Research-Article
Validation of an Empirical Damage Model for Aging and in Vivo Injury of the Murine Patellar Tendon
Andrew A. Dunkman,
Katherine E. Reuther,
Lydia Pathmanathan,
David P. Beason,
David P. Beason
e-mail: dpbeason@gmail.com
McKay Orthopaedic Research Laboratory
,424 Stemmler Hall
,36th Street and Hamilton Walk
,University of Pennsylvania
,Philadelphia, PA 19104
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David E. Birk,
e-mail: dbirk@health.usf.edu
David E. Birk
Department of Molecular Pharmacology and Physiology
,Morsani College of Medicine
,University of South Florida
,12901 Bruce B. Downs Boulevard, MDC 8
,Tampa, FL 33612
e-mail: dbirk@health.usf.edu
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Louis J. Soslowsky
e-mail: soslowsk@upenn.edu
Louis J. Soslowsky
1
McKay Orthopaedic Research Laboratory
,424 Stemmler Hall, 36th Street and Hamilton Walk, University of Pennsylvania
,Philadelphia,PA 19104
e-mail: soslowsk@upenn.edu
1Corresponding author.
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Mark R. Buckley
e-mail: mbuck@upenn.edu
Andrew A. Dunkman
e-mail: andrew.dunkman@gmail.com
Katherine E. Reuther
e-mail: kreuther@seas.upenn.edu
Akash Kumar
e-mail: akkumar@seas.upenn.edu
Lydia Pathmanathan
e-mail: lydia.pathman@gmail.com
David P. Beason
e-mail: dpbeason@gmail.com
McKay Orthopaedic Research Laboratory
,424 Stemmler Hall
,36th Street and Hamilton Walk
,University of Pennsylvania
,Philadelphia, PA 19104
David E. Birk
Department of Molecular Pharmacology and Physiology
,Morsani College of Medicine
,University of South Florida
,12901 Bruce B. Downs Boulevard, MDC 8
,Tampa, FL 33612
e-mail: dbirk@health.usf.edu
Louis J. Soslowsky
McKay Orthopaedic Research Laboratory
,424 Stemmler Hall, 36th Street and Hamilton Walk, University of Pennsylvania
,Philadelphia,PA 19104
e-mail: soslowsk@upenn.edu
1Corresponding author.
Contributed by the Bioengineering Division of ASME for publication in the Journal of Biomechanical Engineering. Manuscript received August 25, 2012; final manuscript received January 25, 2012; accepted manuscript posted February 19, 2013; published online April 2, 2013. Assoc. Editor: Kenneth Fischer.
J Biomech Eng. Apr 2013, 135(4): 041005 (7 pages)
Published Online: April 2, 2013
Article history
Received:
August 25, 2012
Revision Received:
January 25, 2013
Accepted:
February 19, 2013
Citation
Buckley, M. R., Dunkman, A. A., Reuther, K. E., Kumar, A., Pathmanathan, L., Beason, D. P., Birk, D. E., and Soslowsky, L. J. (April 2, 2013). "Validation of an Empirical Damage Model for Aging and in Vivo Injury of the Murine Patellar Tendon." ASME. J Biomech Eng. April 2013; 135(4): 041005. https://doi.org/10.1115/1.4023700
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