Precise geometric reconstruction is a valuable tool in the study of soft tissues biomechanics. Optical methods have been developed to determine the tissue cross section without mechanical contact with the specimen. An adaptation of the laser micrometer developed by Lee and Woo [ASME J. Biomech. Eng., 110 (2), pp. 110–114]. is proposed in which the laser-collimated beam rotates around and moves along a fixed specimen to reconstruct its cross sections and volume. Beam motion is computer controlled to accelerate data acquisition and improve beam positioning accuracy. It minimizes time-dependent shape modifications and increases global reconstruction precision. The technique is also competent for the measurement of immersed collagen matrices.
Issue Section:
Soft Tissue
Keywords:
biological tissues,
biomechanics,
micrometry,
measurement by laser beam,
computerised control,
tissue engineering,
cellular biophysics,
proteins,
molecular biophysics,
laser applications in medicine,
Laser,
Micrometer,
Cross Section,
Profile,
Volume,
Algorithm,
Soft Tissues,
Collagen Matrices
1.
Lanir
, Y.
, Salant
, E. L.
, and Foux
, A.
, 1988
, “Physico-Chemical and Microstructural Changes in Collagen Fiber Bundles Following Stretch in-Vitro
,” Biorheology
, 25
(4
), pp. 591
–603
.2.
Lo´pez Valle
, C. A.
, Auger
, F. A.
, Rompre´
, P.
, Bouvard
, V.
, and Germain
, L.
, 1992
, “Peripheral Anchorage of Dermal Equivalents
,” Br. J. Dermatol.
, 127
(4
), pp. 365
–371
.3.
Basset
, O.
, Gimenez
, G.
, Mestas
, J. L.
, Cathignol
, D.
, and Devonec
, M.
, 1991
, “Volume Measurement by Ultrasonic Transverse or Sagital Cross-Sectional Scanning
,” Ultrasound Med. Biol.
, 17
(3
), pp. 291
–296
.4.
Chan, S. S., Livesay, G. A., Morrow, D. A., and Woo, S. L.-Y., 1995, “The Development of a Low-Cost Laser Reflectance System to Determine the Cross-Sectional Shape and Area of Soft Tissues,” 1995 Advances in Bioengineering, ASME, New York, 31, pp. 123–124.
5.
Ellis
, D. G.
, 1969
, “Cross-Sectional Area Measurements for Tendon Specimens: A Comparison of Several Methods
,” J. Biomech.
, 2
, pp. 175
–186
.6.
Gillis
, C.
, Sharkey
, N.
, Stover
, S. M.
, Pool
, R. R.
, Meagher
, D. M.
, and Willits
, N.
, 1995
, “Ultrasonography as a Method to Determine Tendon Cross-sectional Area
,” Am. J. Vet. Res.
, 56
(10
), pp. 1270
–1274
.7.
Iaconis
, F.
, Steindler
, R.
, and Marinozzi
, G.
, 1987
, “Measurements of Cross-Sectional Area of Collagen Structures (Knee Ligaments) by Means of Optical Method
,” J. Biomech.
, 20
(10
), pp. 1003
–1010
.8.
Lee
, T. Q.
, and Woo
, S. L.-Y.
, 1988
, “A New Method for Determining Cross-Sectional Shape and Area of Soft Tissues
,” ASME J. Biomech. Eng.
, 110
(2
), pp. 110
–114
.9.
Love, C. L., Korvick, D. L., Lanctot, D. R., Agrawal, C. M., and Athanasiou, K. A., 1998, “Design, Validation, and Application of a System to Measure Cross-Sectional Area in the Rat Achilles Tendons,” 17th Southern Biomedical Engineering Conference, pp. 25.
10.
Njus, G. O., and Njus, N. M., 1986, “A Noncontact Method for Determining Cross-Sectional Area of Soft Tissues,” Transactions of the 32nd Meeting, Orthopaedic Research Society, 11, pp. 126.
11.
Noguchi
, M.
, Kitaura
, T.
, Ikoma
, K.
, and Kusaka
, Y.
, 2002
, “A Method of In-Vitro Measurement of the Cross-Sectional Area of Soft Tissues, Using Ultrasonography
,” J. Orthop. Sci.
, 7
(2
), pp. 247
–251
.12.
Race
, A.
, and Amis
, A. A.
, 1996
, “Cross-Sectional Area Measurement of Soft Tissue. A New Casting Method
,” J. Biomech.
, 29
(9
), pp. 1207
–1212
.13.
Shrive
, N. G.
, Lam
, T. C.
, Damson
, E.
, and Frank
, C. B.
, 1988
, “A New Method of Measuring the Cross-Sectional Area of Connective Tissue Structures
,” ASME J. Biomech. Eng.
, 110
(2
), pp. 104
–109
.14.
Vanderby
, Jr., R.
, Masters
, G. P.
, Bowers
, J. R.
, and Graf
, B. K.
, 1991
, “A Device to Measure the Cross-Sectional Area of Soft Connective Tissues
,” IEEE Trans. Biomed. Eng.
, 38
(9
), pp. 1040
–1042
.15.
Woo
, S. L.-Y.
, Danto
, M. I.
, Ohland
, K. J.
, Lee
, T. Q.
, and Newton
, P. O.
, 1990
, “The Use of a Laser Micrometer System to Determine the Cross-Sectional Shape and Area of Ligaments: A Comparative Study With two Existing Methods
,” ASME J. Biomech. Eng.
, 112
(4
), 426
–431
.16.
Harner
, C. D.
, Livesay
, S.
, Kashiwaguchi
, H.
, Fujie
, H.
, Choi
, N. Y.
, and Woo
, S. L.-Y.
, 1995
, “Comparative Study of the Size and Shape of Human Anterior and Posterior Cruciate Ligaments
,” J. Orthop. Res.
, 13
, pp. 429
–434
.17.
Dupuis, D., 2002, “De´shydratation de matrices collage´niques reconstruites in vitro: Effets sur les proprie´te´s me´caniques et histologiques,” Ph.D. thesis, Universite´ Laval, Que´bec.
18.
Goulet, F., Rancourt, D., Cloutier, R., Germain, L., Poole, A. R., and Auger, F. A., 2000, Principles of Tissue Engineering, Academic Press, San Diego, Chap. 50, pp. 711–722.
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