Bio-artificial tissues are being developed as replacements for damaged biologic tissues. Their mechanical properties are critical for load bearing applications. Current testing protocols for bio-artificial tissues vary widely and often do not consider viscoelasticity. Uniaxial stretch tests were performed on fibroblast populated collagen matrices (FPCMs) to determine the influence of specific test protocols on the mechanical behavior. The peak force, hysteresis and shape of the force-stretch curve are affected by the stretch rate, rest period, stretch amplitude and the number and magnitude of preconditioning cycles.
1.
Bell
, E.
, Ivarsson
, B.
, and Merrill
, C.
, 1979
, “Production of a Tissue-Like Structure by Contraction of Collagen Lattices by Human Fibroblasts of Different Proliferative Potential In Vitro
,” Proc. Natl. Acad. Sci. U.S.A.
, 76
, pp. 1274
–1278
.2.
Auger
, F. A.
, Rouabhia
, M.
, Goulet
, F.
, Berthod
, F.
, Moulin
, V.
, and Germain
, L.
, 1998
, “Tissue-Engineered Human Skin Substitutes From Collagen-Populated Hydrated Gels: Clinical and Findamental Applications
,” Med. Biol. Eng. Comput.
, 36
, pp. 801
–812
.3.
Huang
, D.
, Chang
, T. R.
, Aggarwal
, A.
, Lee
, R. C.
, and Ehrlich
, H. P.
, 1993
, “Mechanisms and Dynamics of Mechanical Strengthening in Ligament-Equivalent Fibroblast-Populated Collagen Matrices
,” Ann. Biomed. Eng.
, 21
, pp. 289
–305
.4.
Seliktar
, D.
, Black
, R. A.
, Vito
, R. P.
, and Nerem
, R. M.
, 2000
, “Dynamic Mechanical Conditioning of Collagen-Gel Blood Vessel Constructs Induces Remodeling In Vitro
,” Ann. Biomed. Eng.
, 28
, pp. 351
–362
.5.
Butler
, D. L.
, Goldstein
, S. A.
, and Guilak
, F.
, 2000
, “Functional Tissue Engineering: The Role of Biomechanics
,” J. Biomech. Eng.
, 122
, pp. 570
–575
.6.
Fung, Y. C., 1993, Biomechanics: Mechanical Properties of Living Tissues, Springer-Verlag, New York, pp. 41, 46, 262–263, 270–272, 301.
7.
Fung
, Y. C.
, Fronek
, K.
, and Patitucci
, P.
, 1979
, “Pseudoelasticity of Arteries and the Choice of its Mathematical Expression
,” Am. J. Physiol.
, 237
, pp. H620–H631
H620–H631
.8.
Emery
, J. L.
, Omens
, J. H.
, and McCulloch
, A. D.
, 1997
, “Strain Softening in the Rat Left Ventricular Myocardium
,” J. Biomech. Eng.
, 119
, pp. 6
–12
.9.
Gregersen
, H.
, Emery
, J. L.
, and McCulloch
, A. D.
, 1998
, “History-Dependent Mechanical Behavior of Guinea-Pig Small Intestine
,” Ann. Biomed. Eng.
, 26
, pp. 850
–858
.10.
Wakatsuki
, T.
, Kolodney
, M. S.
, Zahalak
, G. I.
, and Elson
, E. L.
, 2000
, “Cell Mechanics Studied by a Reconstituted Model Tissue
,” Biophys. J.
, 79
, pp. 2353
–2368
.11.
Zahalak
, G.
, Wagenseil
, J.
, Wakatsuki
, T.
, and Elson
, E.
, 2000
, “A Cell-Based Constitutive Relation for Bio-Artificial Tissues
,” Biophys. J.
, 79
, pp. 2369
–2381
.12.
Girton
, T. S.
, Oegema
, T. R.
, and Tranquillo
, R. T.
, 1999
, “Exploiting Glycation to Stiffen and Strengthen Tissue Equivalents for Tissue Engineering
,” J. Biomed. Mater. Res.
, 46
, pp. 87
–92
.13.
Vawter
, D. L.
, Fung
, Y. C.
, and West
, J. B.
, 1978
, “Elasticity of Excised Dog Lung Parenchyma
,” J. Appl. Physiol.
, 45
, pp. 261
–269
.14.
McElhaney
, J. H.
, 1966
, “Dynamic Response of Bone and Muscle Tissue
,” J. Appl. Physiol.
, 21
, pp. 1231
–1236
.15.
Pinto
, J. G.
, and Fung
, Y. C.
, 1973
, “Mechanical Properties of the Heart Muscle in the Passive State
,” J. Biomech.
, 6
, pp. 597
–616
.16.
Kang
, T.
, Resar
, J.
, and Humphrey
, J. D.
, 1995
, “Heat-Induced Changes in the Mechanical Behavior of Passive Coronary Arteries
,” ASME J. Biomech. Eng.
, 117
, pp. 86
–93
.17.
May-Newman
, K.
, and Yin
, F. C. P.
, 1995
, “Biaxial Mechanical Behavior of Excised Porcine Mitral Valve Leaflets
,” Am. J. Physiol.
, 269
, pp. H1319–H1327
H1319–H1327
.18.
Takamizawa
, K.
, and Hayashi
, K.
, 1987
, “Strain Energy Density Function and Uniform Strain Hypothesis for Arterial Mechanics
,” J. Biomech.
, 20
, pp. 7
–17
.19.
Johnson
, M.
, and Beatty
, M.
, 1993
, “The Mullins Effect in Uniaxial Extension and its Influence on the Transverse Vibration of a Rubber String
,” Continuum Mech. Thermodyn.
, 5
, pp. 83
–115
.20.
Kolodney
, M. S.
, and Elson
, E. L.
, 1993
, “Correlation of Myosin Light Chain Phosphorylation With Isometric Contraction of Fibroblasts
,” J. Biol. Chem.
, 268
, pp. 23850
–23855
.21.
Holzapfel
, G. A.
, Gasser
, T. C.
, and Stadler
, M.
, 2002
, “A Structural Model for the Viscoelastic Behavior of Arterial Walls: Continuum Formulation and Finite-Element Analysis
,” European Journal of Mechanics a-Solids
, 21
, pp. 441
–463
.22.
Barocas
, V. H.
, and Tranquillo
, R. T.
, 1997
, “An Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: The Interplay Among Cell Traction, Fibrillar Network Deformation, Fibril Alignment, and Cell Contact Guidance
,” ASME J. Biomech. Eng.
, 119
, pp. 137
–145
.23.
Ozerdem
, B.
, and Tozeren
, A.
, 1995
, “Physical Response of Collagen Gels to Tensile Strain
,” J. Biomech. Eng.
, 117
, pp. 397
–401
.24.
Roeder
, B. A.
, Kokini
, K.
, Sturgis
, J. E.
, Robinson
, J. P.
, and Voytik-Harbin
, S. L.
, 2002
, “Tensile Mechanical Properties of Three-Dimensional Type I Collagen Extracellular Matrices With Varied Microstructure
,” J. Biomech. Eng.
, 124
, pp. 214
–222
.25.
Tower
, T. T.
, Neidert
, M. R.
, and Tranquillo
, R. T.
, 2002
, “Fiber Alignment Imaging During Mechanical Testing of Soft Tissues
,” Ann. Biomed. Eng.
, 30
, pp. 1221
–1233
.26.
Eastwood
, M.
, Porter
, R.
, Khan
, U.
, McGrouther
, G.
, and Brown
, R.
, 1996
, “Quantitative Analysis of Collagen Gel Contractile Forces Generated by Dermal Fibroblast and the Relationship to Cell Morphology
,” J. Cell Physiol.
, 166
, pp. 33
–42
.27.
Eastwood
, M.
, McGrouther
, D. A.
, and Brown
, R. A.
, 1994
, “A Culture Force Monitor for Measurement of Contraction Forces Generated in Human Dermal Fibroblast Cultures: Evidence for Cell-Matrix Mechanical Signalling
,” Biochim. Biophys. Acta
, 1201
, pp. 186
–192
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