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Proc. ASME. SBC2011, ASME 2011 Summer Bioengineering Conference, Parts A and B, 329-330, June 22–25, 2011
Paper No: SBC2011-54027
... using RFP-talin, and GFP-actin. Mechanical coupling and kinetics of assembly of these labeled proteins were measured using time-lapse fluorescent images taken under 60X with a multi-point confocal scanner. Raft marker GM1, Actin, and Talin were observed to sequentially accumulate at probe site...