Epithelial ovarian carcinomas are the fourth leading cause of death in women in the United States among all cancers and the leading cause of death from gynecological malignancies1. The main reason for this high rate of mortality is the inability to properly detect these carcinomas early. Investigations for diagnosing ovarian cancer in early stages have been hindered by two major obstacles: lack of adequate cell models to study different cancer stages and lack of a reliable technique to isolate these cancer cells from peritoneal fluid. In trying to solve the first challenge, Dr. Schmelz and collaborators presented a transformed mouse ovarian surface epithelial (MOSE) cell model by isolating different transitional stages of ovarian cancer as cells progressed from a premalignant nontumorigenic phenotype to a highly aggressive malignant phenotype2, 3. In this model four stages of transformed cells, namely early (MOSE-E), early-intermediate (MOSE-E/I), intermediate (MOSE-I) and late (MOSE-L) cells, were distinguishable3. In the current study, we attempt to solve the second challenge of isolating cancer cells from macrophages and fibroblasts, which are found in the peritoneal fluid. Based on differences in cells’ intrinsic electrical properties, a new cell manipulation technique, contactless dielectrophoresis (cDEP), was implemented.
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Investigating Dielectrophoretic Signature of Mouse Ovarian Surface Epithelial Cells, Macrophages and Fibroblasts
Alireza Salmanzadeh,
Alireza Salmanzadeh
Virginia Tech-Wake Forest University, Blacksburg, VA
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Harsha Kittur,
Harsha Kittur
Virginia Tech-Wake Forest University, Blacksburg, VA
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Michael B. Sano,
Michael B. Sano
Virginia Tech-Wake Forest University, Blacksburg, VA
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Mark A. Stremler,
Mark A. Stremler
Virginia Tech, Blacksburg, VA
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P. Christopher Roberts,
P. Christopher Roberts
Virginia Tech, Blacksburg, VA
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Eva M. Schmelz,
Eva M. Schmelz
Virginia Tech, Blacksburg, VA
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Rafael V. Davalos
Rafael V. Davalos
Virginia Tech-Wake Forest University, Blacksburg, VA
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Alireza Salmanzadeh
Virginia Tech-Wake Forest University, Blacksburg, VA
Harsha Kittur
Virginia Tech-Wake Forest University, Blacksburg, VA
Michael B. Sano
Virginia Tech-Wake Forest University, Blacksburg, VA
Mark A. Stremler
Virginia Tech, Blacksburg, VA
P. Christopher Roberts
Virginia Tech, Blacksburg, VA
Eva M. Schmelz
Virginia Tech, Blacksburg, VA
Rafael V. Davalos
Virginia Tech-Wake Forest University, Blacksburg, VA
Paper No:
SBC2012-80872, pp. 35-36; 2 pages
Published Online:
July 19, 2013
Citation
Salmanzadeh, A, Kittur, H, Sano, MB, Stremler, MA, Roberts, PC, Schmelz, EM, & Davalos, RV. "Investigating Dielectrophoretic Signature of Mouse Ovarian Surface Epithelial Cells, Macrophages and Fibroblasts." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 35-36. ASME. https://doi.org/10.1115/SBC2012-80872
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