Tissue engineering provides a means to create fully functional tissue-equivalents that can grow, repair and remodel in vivo. Our laboratory’s approach to fabricating artery- and heart valve-equivalents utilizes cell-seeded fibrin gels. However, even after 4–5 weeks of static incubation, the mechanical properties of these constructs are below those of native tissue. Previous studies in our laboratory have shown a significant role of mechanical stretching in improving properties of collagen-based tissue constructs (Isenberg and Tranquillo, 2003). We examined the effects of cyclic distention (CD) of cell-seeded fibrin-based tubular constructs (TC) and valve-equivalents (VE) after five weeks of culture. We used human dermal fibroblasts and porcine valve interstitial cells as the cell sources. Circumferential strain amplitudes from 2.5% to 15% were applied to evaluate the effects of CD on remodeling of the TC. We further hypothesized that during long-term conditioning, cells adapt to CD of constant strain amplitude, diminishing the remodeling into tissue. We tested this hypothesis by applying step-wise incremental CD (ICD) from 5%–15% strain amplitude and compared this group to a set of samples subject to CD of constant strain amplitude in this range. Based on the outcome of the cyclic distension study with tubular constructs, we applied CD to VE in a novel bioreactor.
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ASME 2008 Summer Bioengineering Conference
June 25–29, 2008
Marco Island, Florida, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4321-5
PROCEEDINGS PAPER
Controlled Cyclic Stretching of Tissue Engineered Heart Valves: Effects on Mechanical Properties and ECM Organization
Zeeshan H. Syedain,
Zeeshan H. Syedain
University of Minnesota, Minneapolis, MN
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Robert T. Tranquillo
Robert T. Tranquillo
University of Minnesota, Minneapolis, MN
Search for other works by this author on:
Zeeshan H. Syedain
University of Minnesota, Minneapolis, MN
Robert T. Tranquillo
University of Minnesota, Minneapolis, MN
Paper No:
SBC2008-192583, pp. 363-364; 2 pages
Published Online:
March 13, 2014
Citation
Syedain, ZH, & Tranquillo, RT. "Controlled Cyclic Stretching of Tissue Engineered Heart Valves: Effects on Mechanical Properties and ECM Organization." Proceedings of the ASME 2008 Summer Bioengineering Conference. ASME 2008 Summer Bioengineering Conference, Parts A and B. Marco Island, Florida, USA. June 25–29, 2008. pp. 363-364. ASME. https://doi.org/10.1115/SBC2008-192583
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