Alterations in blood flow at early embryonic stages can lead to detrimental remodeling and heart defects, but these structural adaptations are not well understood. We hypothesize that deposition of collagens will be increased as shear stress is increased — leading to a stiffer wall. To test this hypothesis a suture (OTB) was tightened around the outflow tract (OFT) of stage HH18 chick embryos for 24 hours to reduce cross sectional area of the lumen. Sham and OTB embryos were immunostained for collagen I, III, VI and XIV, imaged with confocal microscopy, and staining was quantified by grayscale analysis. Changes in fibril collagens I and III were not observed, however deposition of collagens VI and XIV increased in a degree-of-constriction dependent manner. The observed increase in collagen VI and XIV deposition suggests they play a key role in structural adaptation to increased hemodynamic pressure.
- Bioengineering Division
Hemodynamic Forces Regulate Collagen Deposition in the Embryonic Chicken Outflow Tract
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Rennie, MY, Danilchik, M, Thornburg, KL, & Rugonyi, S. "Hemodynamic Forces Regulate Collagen Deposition in the Embryonic Chicken Outflow Tract." Proceedings of the ASME 2013 Summer Bioengineering Conference. Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments. Sunriver, Oregon, USA. June 26–29, 2013. V01AT17A009. ASME. https://doi.org/10.1115/SBC2013-14299
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