One of the most challenging issues in cryopreservation is to identify new and improved cryoprotective agents (CPAs) or CPA mixtures beyond widely used dimethyl sulfoxide (DMSO). In order to address this challenge, numerous combinations of CPA mixtures need to be prepared, characterized, and screened. The characterization includes thermal properties and phase change behavior during freezing and thawing. For an example, Han et al. [1] performed analysis of the phase change behavior and the thermal properties of phosphate buffers saline (PBS) with various chemical additives (CPAs, AFPs, excess salts) using a differential scanning calorimeter and a cryo-microscope. Considering the number of possible compositions and concentration of CPA mixtures, a high throughput (HTP) screening platform capable of preparing and characterizing array of CPA mixtures is highly desired.
Skip Nav Destination
ASME 2010 Summer Bioengineering Conference
June 16–19, 2010
Naples, Florida, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4403-8
PROCEEDINGS PAPER
High Throughput Characterization of Cryoprotective Agent Mixtures Using an EWOD-Based Digital Microfluidic Device
Sinwook Park,
Sinwook Park
Purdue University, West Lafayette, IN
Search for other works by this author on:
Praveen Kunchala,
Praveen Kunchala
University of Texas at Arlington, Arlington, TX
Search for other works by this author on:
Hyejin Moon,
Hyejin Moon
University of Texas at Arlington, Arlington, TX
Search for other works by this author on:
Bumsoo Han
Bumsoo Han
Purdue University, West Lafayette, IN
Search for other works by this author on:
Sinwook Park
Purdue University, West Lafayette, IN
Praveen Kunchala
University of Texas at Arlington, Arlington, TX
Hyejin Moon
University of Texas at Arlington, Arlington, TX
Bumsoo Han
Purdue University, West Lafayette, IN
Paper No:
SBC2010-19273, pp. 87-88; 2 pages
Published Online:
July 15, 2013
Citation
Park, S, Kunchala, P, Moon, H, & Han, B. "High Throughput Characterization of Cryoprotective Agent Mixtures Using an EWOD-Based Digital Microfluidic Device." Proceedings of the ASME 2010 Summer Bioengineering Conference. ASME 2010 Summer Bioengineering Conference, Parts A and B. Naples, Florida, USA. June 16–19, 2010. pp. 87-88. ASME. https://doi.org/10.1115/SBC2010-19273
Download citation file:
5
Views
Related Proceedings Papers
Related Articles
Thermodynamic Nonequilibrium Phase Change Behavior and Thermal Properties of Biological Solutions for Cryobiology Applications
J Biomech Eng (April,2004)
Application of the Conservation Element and Solution Element Method in Numerical Modeling of Three-Dimensional Heat Conduction With Melting and/or Freezing
J. Heat Transfer (December,2004)
Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope
J. Heat Transfer (September,2015)
Related Chapters
Field Measurements of Frost Penetration into a Landfill Cover that uses a Paper Sludge Barrier
Field Instrumentation for Soil and Rock
Zero-Latency Hybrid Scheduling Scheme for near VoD Services
International Conference on Computer Engineering and Technology, 3rd (ICCET 2011)
Research and Implementation of LCD Driver Based on Embedded Linux
International Conference on Information Technology and Computer Science, 3rd (ITCS 2011)