The annulus fibrosus (AF) of the intervertebral disc is a multi-lamellar fibrocartilage that, together with the nucleus pulposus, confers mechanical support and flexibility to the spine. Function of the AF is predicated on a high degree of structural organization over multiple length scales: aligned collagen fibers reside within each lamella, and the direction of alignment alternates between adjacent lamellae from +30° to −30° with respect to the transverse axis of the spine. Electrospinning permits fabrication of scaffolds consisting of aligned arrays of nanofibers, and has proven effective for directing the alignment of both cells and extracellular matrix (ECM) deposition [1–3]. We recently employed electrospinning to engineer the primary functional unit of the AF, a single lamella [4]. However, it remains a challenge to engineer a multi-lamellar tissue that replicates the cross-ply fiber architecture of the native AF. Moreover, relatively few studies have considered functional properties of engineered AF, and, when measured, tensile properties of these constructs have been inferior to native AF [4]. In this study, mesenchymal stem cells (MSCs) were seeded onto aligned nanofibrous scaffolds organized into bi-lamellar constructs with opposing or parallel fiber orientations, and their functional maturation was evaluated with time. Additionally, we determined a novel role for inter-lamellar ECM in reinforcing the tensile response of bilayers, and confirmed this mechanism by testing acellular bilayers with controllable interface properties.
Skip Nav Destination
ASME 2009 Summer Bioengineering Conference
June 17–21, 2009
Lake Tahoe, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4891-3
PROCEEDINGS PAPER
Mesenchymal Stem Cell Seeded Nanofibrous Laminates Mimic the Multi-Scale Form and Function of the Annulus Fibrosus
Nandan K. Nerurkar,
Nandan K. Nerurkar
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Sounok Sen,
Sounok Sen
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Emily E. Wible,
Emily E. Wible
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Jeffrey B. Stambough,
Jeffrey B. Stambough
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Dawn M. Elliott,
Dawn M. Elliott
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Robert L. Mauck
Robert L. Mauck
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Nandan K. Nerurkar
University of Pennsylvania, Philadelphia, PA
Sounok Sen
University of Pennsylvania, Philadelphia, PA
Emily E. Wible
University of Pennsylvania, Philadelphia, PA
Jeffrey B. Stambough
University of Pennsylvania, Philadelphia, PA
Dawn M. Elliott
University of Pennsylvania, Philadelphia, PA
Robert L. Mauck
University of Pennsylvania, Philadelphia, PA
Paper No:
SBC2009-206812, pp. 417-418; 2 pages
Published Online:
July 19, 2013
Citation
Nerurkar, NK, Sen, S, Wible, EE, Stambough, JB, Elliott, DM, & Mauck, RL. "Mesenchymal Stem Cell Seeded Nanofibrous Laminates Mimic the Multi-Scale Form and Function of the Annulus Fibrosus." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 417-418. ASME. https://doi.org/10.1115/SBC2009-206812
Download citation file:
7
Views
Related Proceedings Papers
Related Articles
Cellular Response to Cyclic Compression of Tissue Engineered Intervertebral Disk Constructs Composed of Electrospun Polycaprolactone
J Biomech Eng (June,2018)
Novel Modeling Approach to Generate a Polymeric Nanofiber Scaffold for Salivary Gland Cells
J. Nanotechnol. Eng. Med (August,2010)
A Novel Device to Quantify the Mechanical Properties of Electrospun Nanofibers
J Biomech Eng (October,2012)
Related Chapters
Part 2, Section II—Materials and Specifications
Companion Guide to the ASME Boiler and Pressure Vessel Code, Volume 1, Third Edition
Part 2, Section II—Materials and Specifications
Companion Guide to the ASME Boiler & Pressure Vessel Code, Volume 1, Second Edition
Section VIII: Division 2–Alternative Rules
Companion Guide to the ASME Boiler & Pressure Vessel Codes, Volume 2, Sixth Edition