The intervertebral disc (IVD) is a unique orthopaedic tissue consisting of at least two cell types: fibroblast-like annulus fibrosus (AF) cells and chondrocyte-like nucleus pulposus (NP) cells. Culture of cells in 3D gel matrices (such as alginate or agarose), maintains the normal morphology and ECM molecule production of chondrocytes for extended periods of time and also allows the application of various forms of mechanical stimulation, such as hydrostatic or compressive loading. In vivo studies have shown IVD cells to be responsive to frequency, duration, and amplitude of mechanical load [1]. IVD literature on mechanobiology uses varying methodologies to apply dynamic loads (compression, hydrostatic forces), with different times of mechanical stimulation, differences in model systems (in vivo, tissue culture, cell culture), species, and ages, and an optimal loading protocol to stimulate extracellular matrix protein accumulation is unknown. The overall goal of this work is to evaluate the potential, and perhaps even feasibility, of mechanical stimulation for extracellular matrix (ECM) regeneration using intervertebral disc cells. Also of interest is whether cells from mature tissue are capable of serving as a potential cell source for future IVD regeneration [2,3].
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ASME 2007 Summer Bioengineering Conference
June 20–24, 2007
Keystone, Colorado, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4798-5
PROCEEDINGS PAPER
Effect of Age and Frequency on Intervertebral Disc Cell Response to Dynamic Compression
Casey L. Korecki,
Casey L. Korecki
University of Vermont, Burlington, VT
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Catherine K. Kuo,
Catherine K. Kuo
National Institutes of Health, Bethesda, MD
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Rocky S. Tuan,
Rocky S. Tuan
National Institutes of Health, Bethesda, MD
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James C. Iatridis
James C. Iatridis
University of Vermont, Burlington, VT
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Casey L. Korecki
University of Vermont, Burlington, VT
Catherine K. Kuo
National Institutes of Health, Bethesda, MD
Rocky S. Tuan
National Institutes of Health, Bethesda, MD
James C. Iatridis
University of Vermont, Burlington, VT
Paper No:
SBC2007-176595, pp. 825-826; 2 pages
Published Online:
March 12, 2014
Citation
Korecki, CL, Kuo, CK, Tuan, RS, & Iatridis, JC. "Effect of Age and Frequency on Intervertebral Disc Cell Response to Dynamic Compression." Proceedings of the ASME 2007 Summer Bioengineering Conference. ASME 2007 Summer Bioengineering Conference. Keystone, Colorado, USA. June 20–24, 2007. pp. 825-826. ASME. https://doi.org/10.1115/SBC2007-176595
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