As one of the key components of Hydrogen Fuel Cell Vehicles (HFCVs), on-board composite hydrogen storage cylinder has developed rapidly in recent years. Hydrogen cycling test is an important means to test the anti-fatigue, bottle mouth leakage, and anti-hydrogen permeability of the tank of the on-board hydrogen storage cylinder. Therefore, a series of technical specifications and standards of different countries have been issued to specify the performance indexes and evaluation methods of hydrogen cycling test of vehicle composite hydrogen storage cylinders. This paper compared and analyzed the differences of hydrogen cycling test methods of vehicle composite hydrogen storage cylinders in various standards from the aspects of test objects, upper and lower limits of circulating pressure, hydrogen charging rate, filling time, gas temperature, hydrogen decompression rate, cycle times and qualified indexes. It was discussed that the relationship between gas temperature in cylinder/hydrogen charging rate/filling time, decompression rate, and qualification criteria of leakage/penetration test at extreme temperature, and it was found that the gas temperature, hydrogen charging rate, and filling time specified in the standard could not provide enough guidance for the practical operation, the upper limit of hydrogen decompression rate was not clear, and the qualified index of leakage/penetration test under extreme temperature needs to be further studied. The results of this study can provide a reference for the hydrogen cycling in Chinese standards for vehicle composite hydrogen storage cylinders in China.