Pedicle screws (PS) are frequently used in medical spinal column fixation. Despite 7 out of 100 pedicle screws fracture inside of the patients’ body and under the claim that lateral bending is the main failure mode, little research has addressed the stress characteristics and the fracture location of the PS under lateral bending. This study focuses on the effect of thread design on the magnitude and location of maximum stress concentration. Four types of thread shapes are considered including V-shape, square-shape, buttress, and reverse buttress. Three-dimensional (3D) finite element (FE) methods are used in this investigation. A load of 150 Newton is applied at the screw head to simulate lateral bending. The models are created in SolidWorks. The 3D FE analysis is performed using the standard coding of ANSYS Workbench 19.1. Based on this study, it is found that the high stress concentration is located at the cortical bone region rather than at the cancellous bone region. Although the general stress patterns are similar, the PS thread shape design and the thread fillet radius may significantly affect on the magnitude and location of maximum stress concentration.