We developed an array device consisting of miniaturized wells and a mechanism of fluid manipulation for cell-free protein synthesis. The array offers high-throughput protein production, matching the format of gene discovery. Each unit in the array is for synthesis of one individual protein and it consists of a tray chamber and a well chamber. The tray chamber is for in vitro protein synthesis reaction, while the well functions as a nutrient reservoir. The tray and well are separated by a dialysis membrane, which is glued to the bottom of the tray. The connection between the tray and the well provides a means to supply nutrients and remove the reaction byproducts. The device was demonstrated by synthesis of green fluorescent protein (GFP). The effectiveness of the device design on the protein production yield has been studied. The resultant advantages due to miniaturization include rapid analysis, less consumption of samples and reagents, and the decrease in the cost of protein synthesis.
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ASME 2006 International Mechanical Engineering Congress and
Exposition
November 5–10, 2006
Chicago, Illinois, USA
Conference Sponsors:
- Microelectromechanical Systems Division
ISBN:
0-7918-4775-6
PROCEEDINGS PAPER
Fabricating a Plastic Microfluidic Device for Protein Synthesis
Carl K. Fredrickson,
Carl K. Fredrickson
University of Florida
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Z. Hugh Fan
Z. Hugh Fan
University of Florida
Search for other works by this author on:
Qian Mei
University of Florida
Carl K. Fredrickson
University of Florida
Andrew Simon
University of Florida
Z. Hugh Fan
University of Florida
Paper No:
IMECE2006-14122, pp. 33-37; 5 pages
Published Online:
December 14, 2007
Citation
Mei, Q, Fredrickson, CK, Simon, A, & Fan, ZH. "Fabricating a Plastic Microfluidic Device for Protein Synthesis." Proceedings of the ASME 2006 International Mechanical Engineering Congress and Exposition. Microelectromechanical Systems. Chicago, Illinois, USA. November 5–10, 2006. pp. 33-37. ASME. https://doi.org/10.1115/IMECE2006-14122
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