Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface phase nucleic acid hybridization, such as modern microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. In this work an electrokinetically controlled poly(dimethylsiloxane) based DNA hybridization microfluidic chip is presented. The electrokinetic delivery technique provides the ability to dispense controlled sample sizes to the hybridization array for quantitative analysis while serving to increase the mass transfer rate and therefore reduce the overall analysis time. An automatic, electrokinetically based, single-nucleotide polymorphism (SNP) discrimination technique (that takes advantage of the combined effects of joule heating, applied potential field and the shear gradients within the double layer field on the thermodynamic stability of the target: probe complex) will also be described for the first time. The clinical utility of the technique will be demonstrated through the detection of genetic markers associated with spinal muscular atrophy, specifically the common C←T mutation in the SMN1 gene.
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ASME 2004 International Mechanical Engineering Congress and Exposition
November 13–19, 2004
Anaheim, California, USA
Conference Sponsors:
- Fluids Engineering Division
ISBN:
0-7918-4709-8
PROCEEDINGS PAPER
A DNA Hybridization Chip With Electrokinetically-Based Single Nucleotide Polymorphism (SNP) Discrimination
Xuezhu Liu,
Xuezhu Liu
University of Toronto at Mississauga
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Ulrich Krull,
Ulrich Krull
University of Toronto at Mississauga
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Dongqing Li
Dongqing Li
University of Toronto
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David Erickson
University of Toronto
Xuezhu Liu
University of Toronto at Mississauga
Roberto Venditti
University of Toronto
Ulrich Krull
University of Toronto at Mississauga
Dongqing Li
University of Toronto
Paper No:
IMECE2004-59320, pp. 271-277; 7 pages
Published Online:
March 24, 2008
Citation
Erickson, D, Liu, X, Venditti, R, Krull, U, & Li, D. "A DNA Hybridization Chip With Electrokinetically-Based Single Nucleotide Polymorphism (SNP) Discrimination." Proceedings of the ASME 2004 International Mechanical Engineering Congress and Exposition. Fluids Engineering. Anaheim, California, USA. November 13–19, 2004. pp. 271-277. ASME. https://doi.org/10.1115/IMECE2004-59320
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