Leukostasis is a life-threatening condition that occurs when leukemia cells accumulate in the vasculature of organs such as the brain and lungs. Recent evidence has shown that leukostasis is not simply due to the physical overcrowding of leukemia cells, as previously thought, but may result from specific mechanical properties of the cells and interactions between cells. Using atomic force microscopy (AFM), we obtained direct measurements of two mechanical properties that are likely involved in this condition: (1) stiffness of individual leukemia cells and (2) non-specific adhesion forces between leukemia cells. We found that myeloid leukemia cells were significantly stiffer than lymphoid leukemia cells. Cell-cell adhesion forces of the cell lines were not found to be statistically different. These results may help to explain the clinical observation that leukostasis occurs in myeloid leukemia at lower white blood cell counts than in lymphoid leukemia.
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ASME 2004 International Mechanical Engineering Congress and Exposition
November 13–19, 2004
Anaheim, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4703-9
PROCEEDINGS PAPER
Contribution of Cell Mechanics to Acute Leukemia
Michael J. Rosenbluth,
Michael J. Rosenbluth
University of California at Berkeley
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Wilbur A. Lam,
Wilbur A. Lam
University of California at Berkeley
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Daniel A. Fletcher
Daniel A. Fletcher
University of California at Berkeley
Search for other works by this author on:
Michael J. Rosenbluth
University of California at Berkeley
Wilbur A. Lam
University of California at Berkeley
Daniel A. Fletcher
University of California at Berkeley
Paper No:
IMECE2004-59881, pp. 395-396; 2 pages
Published Online:
March 24, 2008
Citation
Rosenbluth, MJ, Lam, WA, & Fletcher, DA. "Contribution of Cell Mechanics to Acute Leukemia." Proceedings of the ASME 2004 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. Anaheim, California, USA. November 13–19, 2004. pp. 395-396. ASME. https://doi.org/10.1115/IMECE2004-59881
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