Fluid structure interactions at the cellular level are poorly understood yet they appear to be universal across tissue types and may hold the key to unraveling mechanisms of mechanotransduction at a cellular and subcellular level. Due to practical difficulties in studying cells in situ during normal physiologic activity, cell perfusion chambers have been developed to simulate physiologic fluid flow in vitro. While this approach has obvious advantages for unraveling cell signaling pathways in mechanotransduction, little is known with regard to how well these in vitro flow profiles emulate actual physiologic flow. The purpose of this computational study was to compare the local stress imparted through fluid flow in four cell perfusion chambers. From the computational models, in each chamber, varying velocity components cause the local shear stress imparted to the cells to vary as a function of location, and in fact only a limited number of cells are exposed to target stress. Due to differences in flow regimes between the four chambers, comparison between experimental data obtained using different perfusion chambers may be inappropriate.
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ASME 2004 International Mechanical Engineering Congress and Exposition
November 13–19, 2004
Anaheim, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4703-9
PROCEEDINGS PAPER
Performance Evaluation of Four Cell Flow Chambers: How Well Is Stress Controlled at a Cellular Level?
Eric J. Anderson,
Eric J. Anderson
Case Western Reserve University
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Melissa L. Knothe Tate
Melissa L. Knothe Tate
Case Western Reserve University and Cleveland Clinic Foundation
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Eric J. Anderson
Case Western Reserve University
Adam Sorkin
Cleveland Clinic Foundation
Melissa L. Knothe Tate
Case Western Reserve University and Cleveland Clinic Foundation
Paper No:
IMECE2004-61837, pp. 377-378; 2 pages
Published Online:
March 24, 2008
Citation
Anderson, EJ, Sorkin, A, & Knothe Tate, ML. "Performance Evaluation of Four Cell Flow Chambers: How Well Is Stress Controlled at a Cellular Level?." Proceedings of the ASME 2004 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. Anaheim, California, USA. November 13–19, 2004. pp. 377-378. ASME. https://doi.org/10.1115/IMECE2004-61837
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