In recent years, there are growing interests in the use of free flow isoelectric focusing (FFIEF). In FFIEF, a thin sheath of laminar flow is introduced perpendicular to the direction of the applied electric field for continuous separation of proteins and charged species. This technique is especially useful in microfluidic device since the electrophoretically separated bands do not have to be mobilized for detection or further analysis. In this study, a mathematical model is developed to simulate free flow isoelectric process in microfluidic devices considering electroneutrality and incompressibility of electrolytes. Our mathematical model is based on mass, momentum and charge conservation equations. A finite volume based numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results indicate that pH gradient forms as samples flow downstream and proteins can be separated effectively using this technique. A new design of microfluidic chip is proposed for separation for cardiac troponin I from serum albumin using FFIEF technique.
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ASME 2014 International Mechanical Engineering Congress and Exposition
November 14–20, 2014
Montreal, Quebec, Canada
Conference Sponsors:
- ASME
ISBN:
978-0-7918-4954-5
PROCEEDINGS PAPER
Free Flow Isoelectric Focusing in a Microfluidic Device
Kisoo Yoo,
Kisoo Yoo
Washington State University, Pullam, WA
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Prashanta Dutta,
Prashanta Dutta
Washington State University, Pullam, WA
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Jin Liu
Jin Liu
Washington State University, Pullam, WA
Search for other works by this author on:
Kisoo Yoo
Washington State University, Pullam, WA
Prashanta Dutta
Washington State University, Pullam, WA
Jin Liu
Washington State University, Pullam, WA
Paper No:
IMECE2014-37629, V007T09A063; 6 pages
Published Online:
March 13, 2015
Citation
Yoo, K, Dutta, P, & Liu, J. "Free Flow Isoelectric Focusing in a Microfluidic Device." Proceedings of the ASME 2014 International Mechanical Engineering Congress and Exposition. Volume 7: Fluids Engineering Systems and Technologies. Montreal, Quebec, Canada. November 14–20, 2014. V007T09A063. ASME. https://doi.org/10.1115/IMECE2014-37629
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