A nanoinjection lance array has been developed to inject foreign genetic material into thousands of cells at once using electrophoresis to attract and repel particles to and from the lances. A unique combination of isotropic and anisotropic etch processes are used to fabricate the four million 1 μm by 8 μm solid lances on a 2 cm by 2 cm chip. Initial studies show high cell viability when the lance array is used to pierce through a culture of HeLa cancer cells, often used for genetic research. A mathematical computer model simulating motion of attracted or repelled particles informs the design of the nanoinjection lance array system. The nanoinjection lance array provides an efficient, convenient, and quick way to simultaneously inject thousands of cells for a wide range of genetic research applications.
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ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
August 12–15, 2012
Chicago, Illinois, USA
Conference Sponsors:
- Design Engineering Division
- Computers and Information in Engineering Division
ISBN:
978-0-7918-4504-2
PROCEEDINGS PAPER
Modeling, Fabrication, and Testing of a Nanoinjection Lance Array for Simultaneous Multi-Cell Injections
Nathan C. Toone,
Nathan C. Toone
Brigham Young University, Provo, UT
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Gregory H. Teichert,
Gregory H. Teichert
Brigham Young University, Provo, UT
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Steven J. Brewer,
Steven J. Brewer
Brigham Young University, Provo, UT
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Brian D. Jensen
Brian D. Jensen
Brigham Young University, Provo, UT
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Nathan C. Toone
Brigham Young University, Provo, UT
Gregory H. Teichert
Brigham Young University, Provo, UT
Steven J. Brewer
Brigham Young University, Provo, UT
Brian D. Jensen
Brigham Young University, Provo, UT
Paper No:
DETC2012-70471, pp. 25-32; 8 pages
Published Online:
September 9, 2013
Citation
Toone, NC, Teichert, GH, Brewer, SJ, & Jensen, BD. "Modeling, Fabrication, and Testing of a Nanoinjection Lance Array for Simultaneous Multi-Cell Injections." Proceedings of the ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 5: 6th International Conference on Micro- and Nanosystems; 17th Design for Manufacturing and the Life Cycle Conference. Chicago, Illinois, USA. August 12–15, 2012. pp. 25-32. ASME. https://doi.org/10.1115/DETC2012-70471
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