A method involving the use of magnetic nanoparticles to suppress the cross-reactions in immunoassay is developed. Antibodies are coated onto magnetic nanoparticles. These antibodies bind with target and non-target molecules. Once an alternative-current magnetic field is applied, magnetic nanoparticles oscillate with the magnetic field. The target and non-target molecules attached onto magnetic nanoparticles via antibodies experience a centrifugal force, which is against the association between antibodies and target/non-target molecules. Theoretically, the centrifugal force is proportional to the square of the frequency of the applied magnetic field. Thus, the strength of the centrifugal force can be manipulated by changing the frequency of the applied magnetic field. By well controlling the frequency of applied magnetic field, the centrifugal force can be stronger than the binding between antibodies and non-target molecules, but still weaker than that of target molecules. Consequently, the binding between antibodies and non-target molecules is broken by the centrifugal force.
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ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
August 15–18, 2010
Montreal, Quebec, Canada
Conference Sponsors:
- Design Engineering Division and Computers in Engineering Division
ISBN:
978-0-7918-4412-0
PROCEEDINGS PAPER
Magnetic Assistance for Suppressing Cross-Reactions Between Biomolecules in Immunoassay
Chin-Yih Hong,
Chin-Yih Hong
National Chung Hsing University, Taichung, Taiwan
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Shieh-Yueh Yang,
Shieh-Yueh Yang
MagQu Co., Ltd., Sindian, Taipei, Taiwan
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Herng-Er Horng,
Herng-Er Horng
National Taiwan Normal University, Taipei, Taiwan
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Hong-Chang Yang
Hong-Chang Yang
National Taiwan University, Taipei, Taiwan
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Chin-Yih Hong
National Chung Hsing University, Taichung, Taiwan
Shieh-Yueh Yang
MagQu Co., Ltd., Sindian, Taipei, Taiwan
Herng-Er Horng
National Taiwan Normal University, Taipei, Taiwan
Hong-Chang Yang
National Taiwan University, Taipei, Taiwan
Paper No:
DETC2010-28040, pp. 393-396; 4 pages
Published Online:
March 8, 2011
Citation
Hong, C, Yang, S, Horng, H, & Yang, H. "Magnetic Assistance for Suppressing Cross-Reactions Between Biomolecules in Immunoassay." Proceedings of the ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 4: 12th International Conference on Advanced Vehicle and Tire Technologies; 4th International Conference on Micro- and Nanosystems. Montreal, Quebec, Canada. August 15–18, 2010. pp. 393-396. ASME. https://doi.org/10.1115/DETC2010-28040
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